4PBW
Crystal structure of chicken receptor protein tyrosine phosphatase sigma in complex with TrkC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.420, 93.120, 99.380 |
| Unit cell angles | 73.37, 89.45, 74.23 |
Refinement procedure
| Resolution | 94.960 - 3.050 |
| R-factor | 0.22682 |
| Rwork | 0.226 |
| R-free | 0.23994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.020 | 3.130 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.069 | 0.347 |
| Number of reflections | 51063 | |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 96.3 | 96.6 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.5 | 10% w/v PEG MME 5k, 0.1 M HEPES pH 7, 5% w/v Tacsimate |
