4PBV
Crystal structure of chicken receptor protein tyrosine phosphatase sigma in complex with TrkC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.300, 122.160, 98.610 |
| Unit cell angles | 90.00, 109.79, 90.00 |
Refinement procedure
| Resolution | 92.790 - 2.500 |
| R-factor | 0.21055 |
| Rwork | 0.209 |
| R-free | 0.24701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.790 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 52662 | |
| <I/σ(I)> | 10.7 | 1.6 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 11.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 10% PEG 20k, 20% PEG MME 550, 0.1M bicine/Tris pH8.5, 0.03M sodium nitrate, 0.03M disodium hydrogen phosphate, 0.03M ammonium sulphate |
