4PBT
Crystal structure of the M. jannaschii G2 tRNA synthetase variant bound to 4-trans-cyclooctene-amidopheylalanine (Tco-amF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.640, 101.640, 72.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.900 - 1.900 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zp1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.433 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 71.900 |
High resolution limit [Å] | 1.900 |
Number of reflections | 30390 |
<I/σ(I)> | 11.6 |
Completeness [%] | 100.0 |
Redundancy | 24.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20-22% PEG 300, 5% PEG 8000, 10% glycerol and 100 mM Tris |