4PB6
Feline calicivirus VP1 T=1 virus-like particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9185 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 358.336, 358.336, 358.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.510 - 8.000 |
| R-factor | 0.44232 |
| Rwork | 0.441 |
| R-free | 0.46285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb 3m8l and model builting |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.200 | 8.430 |
| High resolution limit [Å] | 8.000 | 8.000 |
| Rmerge | 0.240 | 1.100 |
| Number of reflections | 14809 | |
| <I/σ(I)> | 3.07 | 0.69 |
| Completeness [%] | 87.2 | 61.5 |
| Redundancy | 5.5 | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | reservoir solution: 7 - 10 % PEG 6000, 0.1 M Hepes pH 7, 0.5 - 1 M LiCl protein in: 20 mM MES pH 6, 200 mM NaCl at 3.4 mg/ml |
