4P9T

Structure of the free form of the N-terminal VH1 domain of monomeric alpha-catenin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-14
Detector	RAYONIX MX300HE
Wavelength(s)	1.0000
Spacegroup name	P 31
Unit cell lengths	68.777, 68.777, 207.965
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.177 - 2.500
R-factor	0.2157
Rwork	0.215
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dov
RMSD bond length	0.008
RMSD bond angle	0.920
Data scaling software	HKL-2000
Phasing software	MrBUMP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500	2.500
Number of reflections	38552
<I/σ(I)>	46.2
Completeness [%]	99.5	100
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG3350, 0.1 M Bis-Tris propane (pH 6.5), 0.2 M Sodium iodide