4P9S
Crystal structure of the mature form of rat DMGDH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.211, 131.442, 171.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.320 |
| R-factor | 0.25388 |
| Rwork | 0.253 |
| R-free | 0.29479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pj5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.444 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 75192 | |
| <I/σ(I)> | 13.7 | 2.3 |
| Completeness [%] | 91.1 | 85.5 |
| Redundancy | 5.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.2 M K/Na-tartrate, 20% PEG3350 |
