4P9E
Crystal structure of dCMP deaminase from the cyanophage S-TIM5 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 78.130, 78.130, 80.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.660 - 2.600 |
| R-factor | 0.21466 |
| Rwork | 0.212 |
| R-free | 0.27163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvv |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.283 |
| Number of reflections | 4840 | |
| <I/σ(I)> | 23 | 8.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8M Malic acid pH 7 |
