4P97
Functional conservation despite structural divergence in ligand-responsive RNA switches
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 60.532, 60.532, 122.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.210 - 1.860 |
| R-factor | 0.21163 |
| Rwork | 0.212 |
| R-free | 0.19682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.560 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 1699 | |
| <I/σ(I)> | 10.78 | 3.67 |
| Completeness [%] | 99.1 | 90.3 |
| Redundancy | 8.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 24% PEG 4000, 10mM Calcium chloride, 200 mM Ammonium Chloride |
