4P96

FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	77
Detector technology	CCD
Collection date	2012-02-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9764
Spacegroup name	P 41
Unit cell lengths	87.480, 87.480, 81.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.560 - 2.200
R-factor	0.1759
Rwork	0.174
R-free	0.20460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((phenix.molecular replacement: 1.8.4-1496))
Refinement software	PHENIX ((phenix.refine: 1.8.4-1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.560	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	31200
<I/σ(I)>	33.79	6.62
Completeness [%]	100.0	100
Redundancy	15.25	15.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.2 M sodium chloride, 0.1 M MES pH 6.5, 10%(w/v) PEG 4000