4P8Q
Crystal Structure of Human Insulin Regulated Aminopeptidase with Alanine in Active Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.323, 256.051, 71.132 |
| Unit cell angles | 90.00, 115.12, 90.00 |
Refinement procedure
| Resolution | 48.420 - 3.020 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.25000 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.420 | 3.190 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.111 | 0.692 |
| Number of reflections | 42874 | |
| <I/σ(I)> | 11.2 | 1.9 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 3000, tris hydrochloride |
