4P8I

Tgl - a bacterial spore coat transglutaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-11
Detector	MAR CCD 165 mm
Wavelength(s)	0.918
Spacegroup name	P 41
Unit cell lengths	97.940, 97.940, 66.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.850 - 1.850
Rwork	0.158
R-free	0.20800
Data scaling software	XDS (31-12-2013)
Phasing software	SHARP (2.8.2)
Refinement software	BUSTER-TNT (2.10)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.850
High resolution limit [Å]	1.850
Number of reflections	47409
<I/σ(I)>	29.53
Completeness [%]	88.6
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	41% (w/V) PEG 400, 0.2 M lithium sulphate, 0.1 M sodum citrate