4P8E
Structure of ribB complexed with substrate (Ru5P) and metal ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.079, 77.090, 97.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.040 |
| R-factor | 0.17844 |
| Rwork | 0.176 |
| R-free | 0.23216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g58 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.147 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.065 | 0.258 |
| Number of reflections | 25330 | |
| <I/σ(I)> | 23.1 | 6.4 |
| Completeness [%] | 98.3 | 92.7 |
| Redundancy | 7.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 293 | PEG3350, Na2HPO4 |
