4P7U

Extracellular domain of type II Transforming Growth Factor Beta receptor in complex with NDSB-201

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	33.574, 40.463, 75.837
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.439 - 1.502
R-factor	0.174
Rwork	0.171
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m9z
RMSD bond length	0.007
RMSD bond angle	1.181
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.100	22.100	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.054	0.041	0.419
Total number of observations	104863
Number of reflections	17064
<I/σ(I)>	11.9
Completeness [%]	99.5	99.9	98.5
Redundancy	6.1	5.7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	100 mM sodium citrate, 30% PEG 2000