4P7B

Crystal structure of S. typhimurium peptidyl-tRNA hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	62.097, 64.925, 110.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.876 - 1.600
R-factor	0.1749
Rwork	0.174
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fyj
RMSD bond length	0.012
RMSD bond angle	1.285
Refinement software	PHENIX ((phenix.refine: dev_1458))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.880
High resolution limit [Å]	1.600
Number of reflections	59556
<I/σ(I)>	16.57
Completeness [%]	99.8
Redundancy	5.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	citric acid, Bis-Tris propane, glycerol, sucrose, PEG6000