4P60
Structure of the N-terminal domain of the human mitochondrial aspartate/glutamate carrier Aralar in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 103.520, 103.520, 185.559 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.500 - 2.400 |
| R-factor | 0.2477 |
| Rwork | 0.245 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p5x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.650 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.503 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 1.242 |
| Number of reflections | 23734 | |
| <I/σ(I)> | 19 | 1.7 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 9.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.3M sodium acetate, 0.1M Tris, 8% PEG 20000, 8% PEG 550 MME |
