4P5W
Structure of the N- and C-terminal domain fusion of the human mitochondrial aspartate/glutamate carrier Citrin in the calcium-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.149, 106.167, 117.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.080 - 2.400 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22690 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.785 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.084 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 | 0.685 |
| Number of reflections | 34132 | |
| <I/σ(I)> | 13.2 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.2M sodium malonate, 20% PEG 3350 |
