4P5O

Structure of an RBX1-UBC12~NEDD8-CUL1-DCN1 complex: a RING-E3-E2~ubiquitin-like protein-substrate intermediate trapped in action

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	CCD
Collection date	2013-07-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97912
Spacegroup name	P 1 21 1
Unit cell lengths	89.105, 129.606, 119.940
Unit cell angles	90.00, 111.79, 90.00

Refinement procedure

Resolution	42.133 - 3.107
R-factor	0.2303
Rwork	0.227
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.236
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Number of reflections	45594
<I/σ(I)>	13.46
Completeness [%]	99.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11% PEG3350, 0.2M ammonium citrate, 10mM ATP