4P4P

Crystal structure of Leishmania infantum polymerase beta: Nick complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 65 2 2
Unit cell lengths	106.497, 106.497, 159.609
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.303 - 2.297
R-factor	0.1948
Rwork	0.191
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bpy
RMSD bond length	0.006
RMSD bond angle	1.045
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.297
Number of reflections	24544
<I/σ(I)>	20.9
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6 and 12.5% PEG 4000