4P3Y

Crystal structure of Acinetobacter baumannii DsbA in complex with EF-Tu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	74.318, 78.190, 122.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.949 - 2.154
R-factor	0.1721
Rwork	0.170
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
RMSD bond length	0.008
RMSD bond angle	1.170
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.320	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.065	0.596
Number of reflections	39200
<I/σ(I)>	13.7	2.5
Completeness [%]	99.4	98.2
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	KCl, PEG 3350