4P3U
Crystal structure of the bacterial A1408U-mutant ribosomal decoding site (C2 form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.380, 32.730, 80.990 |
| Unit cell angles | 90.00, 117.06, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.000 |
| Rwork | 0.295 |
| R-free | 0.30240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.287 |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.600 | 25.600 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.430 | 3.000 |
| Rmerge | 0.091 | 0.037 | 0.377 |
| Total number of observations | 26787 | 2525 | 2635 |
| Number of reflections | 7533 | ||
| <I/σ(I)> | 7.1 | 24.3 | 2.2 |
| Completeness [%] | 99.4 | 95.3 | 100 |
| Redundancy | 3.53 | 3.21 | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium Cacodylate, Spermine tetrahydrochloride, PEG3350, Sodium Cacodylate |
