4P3T
Crystal structure of the bacterial A1408C-mutant ribosomal decoding site
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.91992, 0.92005, 0.90660, 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.437, 40.475, 41.272 |
| Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.248 |
| R-free | 0.27060 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.881 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.200 | 40.200 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.039 | 0.026 | 0.295 |
| Total number of observations | 102374 | 8105 | 10263 |
| Number of reflections | 14089 | ||
| <I/σ(I)> | 19.2 | 55.5 | 4.9 |
| Completeness [%] | 98.4 | 87.4 | 100 |
| Redundancy | 7.21 | 6.18 | 7.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium Cacodylate (pH7), Spermine tetrahydrochloride, MPD, Ammonium Chloride |
