4P2K
Structure of Ephrin type-A receptor 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.885, 74.796, 76.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.248 - 1.499 |
R-factor | 0.1737 |
Rwork | 0.173 |
R-free | 0.18980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.067 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.079 | 0.072 | 0.176 |
Total number of observations | 358821 | ||
Number of reflections | 50533 | ||
<I/σ(I)> | 26.1 | ||
Completeness [%] | 99.4 | 90.8 | 100 |
Redundancy | 7.1 | 6.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M Ammonium citrate tribasic pH7.0, 20% PEG3350 |