4P1W
Crystal structure of Atg13(17BR)-Atg17-Atg29-Atg31 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 148.348, 63.989, 184.600 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 44.260 - 3.200 |
| R-factor | 0.26058 |
| Rwork | 0.257 |
| R-free | 0.29251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hpq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.341 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.108 | |
| Number of reflections | 54575 | |
| <I/σ(I)> | 8.6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 8% PEGMME 5000, 0.1M TrisHCl |
