4P1T
Crystal structure of the DBL3X-DBL4epsilon double domain from the extracellular part of VAR2CSA PfEMP1 from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-27 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.794, 129.336, 64.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.160 - 2.900 |
| R-factor | 0.207 |
| Rwork | 0.203 |
| R-free | 0.27600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.160 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.263 | 0.936 |
| Number of reflections | 17568 | |
| <I/σ(I)> | 6.8 | 0.8 |
| Completeness [%] | 92.1 | 92.1 |
| Redundancy | 6.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 18% PEG 3000, 100 mM Bis-Tris buffer, 300 mM sodium chloride |
