4P1M
The structure of Escherichia coli ZapA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 54.100, 54.100, 328.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.381 - 1.950 |
R-factor | 0.2263 |
Rwork | 0.224 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w2e |
RMSD bond length | 0.006 |
RMSD bond angle | 0.996 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.840 | |
Number of reflections | 22080 | |
<I/σ(I)> | 19.4 | 2.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | ammonium sulfate, lithium sulfate, sodium citrate. |