4P1L

Crystal structure of a trap periplasmic solute binding protein from chromohalobacter salexigens dsm 3043 (csal_2479), target EFI-510085, with bound d-glucuronate, spg i213

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	I 21 3
Unit cell lengths	158.120, 158.120, 158.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.357 - 1.700
R-factor	0.1447
Rwork	0.143
R-free	0.18110
RMSD bond length	0.011
RMSD bond angle	1.228
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	111.808	42.259	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge		0.033	0.630
Rmeas	0.106
Rpim	0.023	0.007	0.213
Total number of observations	1370709	50830	88714
Number of reflections	71263
<I/σ(I)>	20.5	50.5	3
Completeness [%]	99.1	99.8	93.9
Redundancy	19.2	21.3	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	298	Protein (35.6 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 10 mM D-Glucuronate); Reservoir (0.2M Lithium Sulfate 0.1 M CAPS pH 10.5 2.0 M Ammonium Sulfate ); Cryoprotection (20% glycerol + Reservoir