4P0R

human Mus81-Eme1-3'flap DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	103
Detector technology	CCD
Collection date	2013-09-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	221.757, 135.300, 102.889
Unit cell angles	90.00, 113.27, 90.00

Refinement procedure

Resolution	34.862 - 6.501
R-factor	0.2141
Rwork	0.210
R-free	0.29540
RMSD bond length	0.012
RMSD bond angle	1.689
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	6.460
Number of reflections	7162
<I/σ(I)>	35
Completeness [%]	99.6
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	MPD, Sodium acetate, DTT