4P0P

Crystal structure of Human Mus81-Eme1 in complex with 5'-flap DNA, and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	103
Detector technology	CCD
Collection date	2013-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	85.023, 226.499, 52.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.028 - 2.800
R-factor	0.2232
Rwork	0.222
R-free	0.24310
RMSD bond length	0.004
RMSD bond angle	0.666
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.750
Number of reflections	27032
<I/σ(I)>	17.6
Completeness [%]	96.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Tris, Ethanol