4P0M

Crystal structure of an evolved putative penicillin-binding protein homolog, Rv2911, from Mycobacterium tuberculosis

Replaces:  2BCF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97964
Spacegroup name	H 3 2
Unit cell lengths	75.126, 75.126, 230.435
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.131 - 2.000
R-factor	0.1739
Rwork	0.173
R-free	0.19870
RMSD bond length	0.007
RMSD bond angle	1.100
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.144	0.567
Number of reflections	32821
<I/σ(I)>	7.7	3.8
Completeness [%]	100.0	100
Redundancy	14.9	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	PEG 5000, AMMONIUS SULFATE, SODIUM CHLORIDE, TRIS, CITRATE