4P0L
Crystal Structure of Double Loop-Swapped Interleukin-36Ra With Additional Point Mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 42.773, 42.773, 119.794 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.000 - 1.550 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1md6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.436 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.065 | 0.735 |
| Rpim | 0.022 | 0.250 |
| Total number of observations | 183258 | 8548 |
| Number of reflections | 19268 | |
| <I/σ(I)> | 20.5 | 2.8 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 9.5 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | 30% (w/v) PEG5000MME, 0.1 M NaAcetate, pH 5.3 |
