4P0D

The T6 backbone pilin of serotype M6 Streptococcus pyogenes has a modular three-domain structure decorated with variable loops and extensions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	110
Detector technology	CCD
Collection date	2013-03-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 63
Unit cell lengths	104.240, 104.240, 84.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.2111
Rwork	0.209
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k8w
RMSD bond length	0.008
RMSD bond angle	1.256
Data reduction software	XDS (0.2.8)
Data scaling software	SCALA (2.5.2)
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.480	24.480	1.940
High resolution limit [Å]	1.900	8.910	1.900
Rmerge	0.152	0.051	0.939
Rpim	0.036	0.012	0.229
Total number of observations	665673	7733	27669
Number of reflections	36565
<I/σ(I)>	16.6	44.3	3.8
Completeness [%]	89.1	95.2	57.9
Redundancy	18.2	19.1	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291	2.2 M NaKPO4 pH 6.4, 2 % PEG 400, 0.1 M imidazole, 20 mM Imidazole