4P0C

Crystal Structure of NHERF2 PDZ1 Domain in Complex with LPA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.12719
Spacegroup name	P 1 21 1
Unit cell lengths	26.360, 40.250, 37.100
Unit cell angles	90.00, 107.42, 90.00

Refinement procedure

Resolution	24.208 - 1.339
R-factor	0.1466
Rwork	0.145
R-free	0.17690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	20CS
RMSD bond length	0.007
RMSD bond angle	1.191
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.210
High resolution limit [Å]	1.170
Rmerge	0.045
Number of reflections	24020
<I/σ(I)>	15.3
Completeness [%]	95.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM sodium acetate, 0.2 M ammonium acetate