4P0B
Crystal structure of HOIP PUB domain in complex with OTULIN PIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-05-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 48.162, 48.162, 267.713 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.904 - 2.700 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.26960 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.695 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.160 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 9674 |
| <I/σ(I)> | 23.8 |
| Completeness [%] | 100.0 |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 7.5 |
