4P09
Crystal structure of HOIP PUB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-03-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.920, 47.920, 152.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.722 - 1.700 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.23030 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.802 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.900 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 20365 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 99.0 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 8.5 |
