4OZ7
Methanobactin production by methanotrophic bacteria and their structural diversity from Methylosinus strains: Insights into copper release
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 36.720, 39.420, 40.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.160 - 1.650 |
| R-factor | 0.22216 |
| Rwork | 0.221 |
| R-free | 0.25070 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.859 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.160 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.087 | 0.342 |
| Number of reflections | 3677 | |
| <I/σ(I)> | 11.3 | 3.4 |
| Completeness [%] | 99.0 | 94.1 |
| Redundancy | 6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M Sodium Malonate, 100 mM Hepes pH 7.0, 0.5% v/v Jeffamine ED-2001, 0.5% w/v Octyl-beta-D-glucoside, Crystal were cryoprotected with the above condition supplemented with 20% glycerol. |
