4OYV
Crystal structure of MltF from Pseudomonas aeruginosa complexed with leucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.616, 82.495, 89.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.200 - 2.310 |
| R-factor | 0.2314 |
| Rwork | 0.228 |
| R-free | 0.30400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.595 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.040 | 2.430 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.092 | 0.175 |
| Rmeas | 0.110 | |
| Rpim | 0.059 | |
| Total number of observations | 65955 | |
| Number of reflections | 17631 | |
| <I/σ(I)> | 7 | 4.9 |
| Completeness [%] | 97.5 | 94.7 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 0.2 M MgCl2, 20% (W/V) PEG 8000. |
