4OYK

Structure of HOIP PUB domain bound to OTULIN PIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 61
Unit cell lengths	64.049, 64.049, 172.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.468 - 2.000
R-factor	0.2024
Rwork	0.201
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oyj
RMSD bond length	0.002
RMSD bond angle	0.613
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.470	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.129	0.604
Number of reflections	26916
<I/σ(I)>	5.9	2
Completeness [%]	99.9	99.8
Redundancy	4.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	295	32% PEG 6000, 1M LiCl, 100 mM Tris pH 8.4