4OY5

0.89 Angstrom resolution crystal structure of (Gly-Pro-Hyp)10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-13
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.7293
Spacegroup name	P 1 21 1
Unit cell lengths	13.860, 26.150, 19.880
Unit cell angles	90.00, 105.43, 90.00

Refinement procedure

Resolution	19.160 - 0.890
R-factor	0.06773
Rwork	0.067
R-free	0.07732
Structure solution method	AB INITIO PHASING
RMSD bond length	0.047
RMSD bond angle	4.406
Phasing software	SHELXDE (2013/1)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.190	0.910
High resolution limit [Å]	0.890	0.890
Number of reflections	10270
<I/σ(I)>	54.7	25.4
Completeness [%]	96.5	93.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	22% PEG400, 5% acetic acid