4OX9

Crystal structure of the aminoglycoside resistance methyltransferase NpmA bound to the 30S ribosomal subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	403.520, 403.520, 176.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.715 - 3.804
R-factor	0.234
Rwork	0.233
R-free	0.25680
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1j5e
RMSD bond length	0.008
RMSD bond angle	1.292
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.900
High resolution limit [Å]	3.800	16.990	3.800
Rmerge	0.120	0.045	1.438
Rmeas	0.128	0.048	1.525
Total number of observations	1266264
Number of reflections	142385	1693	10404
<I/σ(I)>	13.22	41.58	1.43
Completeness [%]	99.8	94	99.9
Redundancy	8.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	14% MPD, 0.2 M KCL , 75 mM NH4Cl, 15 mM MgCl2 , 0.1M MES
2	VAPOR DIFFUSION, HANGING DROP	7	277	14% MPD, 0.2 M KCL , 75 mM NH4Cl, 15 mM MgCl2 , 0.1M MES