4OX6

Structure of Synechococcus elongatus PCC 7942 CcmK4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	P 63
Unit cell lengths	67.452, 67.452, 63.351
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.726 - 1.337
R-factor	0.1682
Rwork	0.167
R-free	0.18940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a10
RMSD bond length	0.020
RMSD bond angle	1.792
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.726	1.380
High resolution limit [Å]	1.337	1.337
Rmerge	0.045	0.723
Number of reflections	34786
<I/σ(I)>	28.33	1.63
Completeness [%]	93.8	63.4
Redundancy	9.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MgCl2, 0.1 M sodium citrate pH 5.0, 15 % (w/v) PEG 4000