4OWX

Structural basis of SOSS1 in complex with a 12nt ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	98.630, 162.520, 65.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.807 - 2.300
R-factor	0.1846
Rwork	0.182
R-free	0.22580
RMSD bond length	0.008
RMSD bond angle	1.119
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.810
High resolution limit [Å]	2.300
Number of reflections	47747
<I/σ(I)>	11.4
Completeness [%]	99.8
Redundancy	7.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	16% PEG3350, 0.1M MES pH6.0, 0.1M ammonium formate