4OWW

Structural basis of SOSS1 in complex with a 35nt ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	82.350, 90.970, 111.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.959 - 2.300
R-factor	0.1818
Rwork	0.180
R-free	0.21830
RMSD bond length	0.005
RMSD bond angle	0.928
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	37719
<I/σ(I)>	7.8
Completeness [%]	99.8
Redundancy	5.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% PEG2000 MME, 0.1M MES pH6.0, 0.05M sodium chloride.