4OWP
Crystal structure of rpn11 in a heterodimer complex with rpn8, representing the active portion of the proteasome lid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-21 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.388, 70.388, 199.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.763 - 2.350 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.077 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.138 | 0.057 | 0.810 |
| Total number of observations | 362134 | ||
| Number of reflections | 21697 | ||
| <I/σ(I)> | 8.2 | 1.72 | |
| Completeness [%] | 98.6 | 98.7 | 88.4 |
| Redundancy | 16.7 | 15.1 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 5000, 5% Tacsimate, 0.1 M Hepes pH 7.0 |
