4OWH
PtBr6 binding to HEWL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.663, 78.663, 36.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.620 - 1.484 |
| R-factor | 0.19171 |
| Rwork | 0.190 |
| R-free | 0.22292 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.969 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.620 | 1.520 |
| High resolution limit [Å] | 1.484 | 1.484 |
| Rmerge | 0.059 | 0.207 |
| Number of reflections | 34066 | |
| <I/σ(I)> | 30.8 | 1.9 |
| Completeness [%] | 94.4 | 30.7 |
| Redundancy | 18.7 | 0.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.7 | 295 | HEWL (60 mg) was dissolved in 0.04 M acetate buffer, pH 4.7 (1 mL) and 10% NaCl (1 mL) was added to the solution. HEWL crystals were soaked for 24hrs in a solution of K2PtBr6 (10mM) |
