4OWG

Crystal structure of rabbit muscle triosephosphate isomerase-PEP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	36.848, 72.071, 161.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.080 - 1.550
R-factor	0.169
Rwork	0.167
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r2r
RMSD bond length	0.020
RMSD bond angle	1.905
Data reduction software	MOSFLM (7.0.9)
Phasing software	PHASER (2.5.5)
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.080
High resolution limit [Å]	1.550
Number of reflections	59113
<I/σ(I)>	8
Completeness [%]	93.4
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES pH: 6.5, 25% PEG 8000