4OWD
Crystal structure of MltF from Pseudomonas aeruginosa complexed with cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.601, 82.618, 96.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.760 - 2.210 |
| R-factor | 0.1943 |
| Rwork | 0.192 |
| R-free | 0.23820 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.909 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.686 | 2.320 |
| High resolution limit [Å] | 1.878 | 2.210 |
| Rmerge | 0.040 | 0.079 |
| Rmeas | 0.045 | |
| Rpim | 0.020 | |
| Total number of observations | 95983 | |
| Number of reflections | 95977 | |
| <I/σ(I)> | 22.7 | 11.4 |
| Completeness [%] | 93.2 | 94.9 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 0.2 M MgCl2, 20% (w/v) PEG8000 |
