4OVU

Crystal Structure of p110alpha in complex with niSH2 of p85alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	114.692, 116.205, 149.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.790 - 2.960
R-factor	0.1952
Rwork	0.191
R-free	0.27150
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2rd0
RMSD bond length	0.011
RMSD bond angle	1.689
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.010
High resolution limit [Å]	2.960	8.020	2.960
Rmerge	0.068	0.025	0.686
Total number of observations	300309
Number of reflections	41914
<I/σ(I)>	15.5
Completeness [%]	99.6	94.6	100
Redundancy	7.2	6.5	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	NaFormate