4OUR
Crystal structure of Arabidopsis thaliana phytochrome B photosensory module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97896 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 127.502, 127.502, 300.772 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.565 - 3.400 |
R-factor | 0.2439 |
Rwork | 0.242 |
R-free | 0.26980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vea |
RMSD bond length | 0.002 |
RMSD bond angle | 0.570 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.590 |
High resolution limit [Å] | 3.400 | 5.400 | 3.400 |
Number of reflections | 35082 | ||
Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | magnesium sulfate, polyethylene glycol 3340, glycerol, bistris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |