4OTM

Crystal structure of the C-terminal domain from yeast GCN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.00587
Spacegroup name	P 21 21 2
Unit cell lengths	47.126, 118.896, 46.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.138 - 1.950
R-factor	0.225
Rwork	0.222
R-free	0.27480
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.071
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.040	0.025	0.260
Number of reflections	18888
<I/σ(I)>	23.6
Completeness [%]	94.4	80.9	81.9
Redundancy	3.9	4.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M Bis-Tris, 21-28% (w/v) polyethylene glycol 2000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K