4OT9

crystal structure of the C-terminal domain of p100/NF-kB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	170.81
Detector technology	CCD
Collection date	2011-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	196.670, 196.670, 68.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.570 - 3.350
R-factor	0.233
Rwork	0.233
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MERLOT
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	50.000	3.460
High resolution limit [Å]	3.340	7.190	3.340
Rmerge	0.067	0.028	0.590
Number of reflections	10842
<I/σ(I)>	14.7	30	4
Completeness [%]	92.0	95	99
Redundancy	9.4	8.4	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% PEG 4000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K